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Imaging drought stress in plants

Our knowledge of species drought tolerance is significantly limited yet critical in predicting how plants will respond to a rapidly changing climate. New optical techniques for visualising the effect of water stress provide the foundation for a new generation of tools to assess plant drought tolerance.

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OpenSourceOV is an Open Source project that encourages community involvement.

From electronics to optics to beta-testing, a range of expertise is required to develop and improve the resources you'll find on the site. If you'd like to get involved then head on over to contributing for more information.

Contributing

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How does it work?

Capture

Capture images of a leaf or stem over time using a microscope, off-the-shelf document scanner, or 3D-printed clamp.

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Process

Use the OpenSourceOV plugins and guides to extract embolism events from the captured sequence of images.

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Analyze

Use our detailed guide to analyse the data and present the cumulative effect of increasing drought stress as an optical vulnerability curve.

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remote_transfer

Remotely transferring images from the Pi

By Chris Lucani -

Instead of transferring captured images from the Pi using a USB stick it’s far more efficient to remotely transfer them over a network connection. First, make sure your Pi and the computer you want to transfer the files to (the local computer) are connected to the same network. Most mobile phones have a ‘Mobile Hotspot’…

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Cavicam

Commercial “Cavicam” now available!

By Chris Lucani -

A commercial version of the OSOV clamp is now available. The “Cavicam” is a small, compact time-lapse camera based on the Raspberry Pi platform that can be used for imaging xylem and for optical dendrometry.

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Cavitation visually resolved using OVT matches cavitation resolved with microCT.

Xylem Embolism Spreads by Single-Conduit Events in Three Dry Forest Angiosperm Stems

Xylem cavitation resulting in air embolism is a major cause of plant death during drought, yet the spread of embolism throughout the plant water transport system is poorly understood. Our study used optical visualization and X-ray microcomputed tomography imaging to capture the spread of emboli in stems of three drought-resistant angiosperm trees: Drooping she-oak (Allocasuarina…

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Eucryphia lucida leaf 1

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