At this point you should have a sequence of high resolution images of the xylem. If you don’t, head over to capture for information and resources. More information about the technique and OpenSourceOV project in general can be found in the overview.

Processing the captured sequences involves extracting the differences between pairs of images in the sequence, removing noise and any artefacts (such as a insect walking across the sample!), and quantifying the degree of embolism in each image.

Instructions for processing can be found below. Note that this will take you to Github where all the written resources are hosted and managed. Github is a tool for collaboration, and OpenSourceOV is an Open Source project which means anyone can contribute to improve any of the resources – even if that means correcting a spelling mistake. Check out the contributing page for more information.



Processing Instructions


ImageJ Toolbox


Help improve these resources by contributing to the project.


Integrating image capture and processing with 3rd party processes (or… how to build your own Caviconsole GUI!)

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Combined Stacks_v3-1 Play

Acacia dealbata
Stem xylem conduits embolising in Acacia dealbata. Comparison of original, difference and processed stacks. Captured using the new hand-lens clamp.